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rabbit mab against total rap1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit mab against total rap1
    FIGURE 6. Expression of FPPS and <t>Rap1</t> in monocytes and neutro- phils. Highly purified monocytes and neutrophils were incubated in me- dium alone or in the presence of 2, 10, or 50 mM of ZOL. After 3 h, the cells were washed with culture medium and incubated overnight in fresh medium. Cells were collected, washed with PBS, and lysed. Five micro- grams of cellular lysates were separated by SDS-PAGE. Proteins were transferred to nitrocellulose followed by immunoblot with anti-Rap1A/B mAb (clone 26B4) to detect total Rap1, with polyclonal Ab C-17 to detect unprenylated Rap1A (uRap1A), or anti-FPPS polyclonal Ab and appro- priate HRP-conjugated secondary Ab. Blots were stripped and GAPDH served as a loading control. Comparable results were obtained in a second independent experiment.
    Rabbit Mab Against Total Rap1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit mab against total rap1/product/Cell Signaling Technology Inc
    Average 93 stars, based on 44 article reviews
    rabbit mab against total rap1 - by Bioz Stars, 2026-06
    93/100 stars

    Images

    1) Product Images from "Butyrophilin 3A/CD277-Dependent Activation of Human γδ T Cells: Accessory Cell Capacity of Distinct Leukocyte Populations."

    Article Title: Butyrophilin 3A/CD277-Dependent Activation of Human γδ T Cells: Accessory Cell Capacity of Distinct Leukocyte Populations.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1600913

    FIGURE 6. Expression of FPPS and Rap1 in monocytes and neutro- phils. Highly purified monocytes and neutrophils were incubated in me- dium alone or in the presence of 2, 10, or 50 mM of ZOL. After 3 h, the cells were washed with culture medium and incubated overnight in fresh medium. Cells were collected, washed with PBS, and lysed. Five micro- grams of cellular lysates were separated by SDS-PAGE. Proteins were transferred to nitrocellulose followed by immunoblot with anti-Rap1A/B mAb (clone 26B4) to detect total Rap1, with polyclonal Ab C-17 to detect unprenylated Rap1A (uRap1A), or anti-FPPS polyclonal Ab and appro- priate HRP-conjugated secondary Ab. Blots were stripped and GAPDH served as a loading control. Comparable results were obtained in a second independent experiment.
    Figure Legend Snippet: FIGURE 6. Expression of FPPS and Rap1 in monocytes and neutro- phils. Highly purified monocytes and neutrophils were incubated in me- dium alone or in the presence of 2, 10, or 50 mM of ZOL. After 3 h, the cells were washed with culture medium and incubated overnight in fresh medium. Cells were collected, washed with PBS, and lysed. Five micro- grams of cellular lysates were separated by SDS-PAGE. Proteins were transferred to nitrocellulose followed by immunoblot with anti-Rap1A/B mAb (clone 26B4) to detect total Rap1, with polyclonal Ab C-17 to detect unprenylated Rap1A (uRap1A), or anti-FPPS polyclonal Ab and appro- priate HRP-conjugated secondary Ab. Blots were stripped and GAPDH served as a loading control. Comparable results were obtained in a second independent experiment.

    Techniques Used: Expressing, Incubation, SDS Page, Western Blot, Control



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    Image Search Results


    FIGURE 6. Expression of FPPS and Rap1 in monocytes and neutro- phils. Highly purified monocytes and neutrophils were incubated in me- dium alone or in the presence of 2, 10, or 50 mM of ZOL. After 3 h, the cells were washed with culture medium and incubated overnight in fresh medium. Cells were collected, washed with PBS, and lysed. Five micro- grams of cellular lysates were separated by SDS-PAGE. Proteins were transferred to nitrocellulose followed by immunoblot with anti-Rap1A/B mAb (clone 26B4) to detect total Rap1, with polyclonal Ab C-17 to detect unprenylated Rap1A (uRap1A), or anti-FPPS polyclonal Ab and appro- priate HRP-conjugated secondary Ab. Blots were stripped and GAPDH served as a loading control. Comparable results were obtained in a second independent experiment.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Butyrophilin 3A/CD277-Dependent Activation of Human γδ T Cells: Accessory Cell Capacity of Distinct Leukocyte Populations.

    doi: 10.4049/jimmunol.1600913

    Figure Lengend Snippet: FIGURE 6. Expression of FPPS and Rap1 in monocytes and neutro- phils. Highly purified monocytes and neutrophils were incubated in me- dium alone or in the presence of 2, 10, or 50 mM of ZOL. After 3 h, the cells were washed with culture medium and incubated overnight in fresh medium. Cells were collected, washed with PBS, and lysed. Five micro- grams of cellular lysates were separated by SDS-PAGE. Proteins were transferred to nitrocellulose followed by immunoblot with anti-Rap1A/B mAb (clone 26B4) to detect total Rap1, with polyclonal Ab C-17 to detect unprenylated Rap1A (uRap1A), or anti-FPPS polyclonal Ab and appro- priate HRP-conjugated secondary Ab. Blots were stripped and GAPDH served as a loading control. Comparable results were obtained in a second independent experiment.

    Article Snippet: Western blots were separately analyzed using rabbit polyclonal Ab against FPPS (PA5-28228; Thermo Fisher Scientific), goat polyclonal Ab against unprenylated Rap 1A (C-17, sc-1482; Santa Cruz Biotechnology, Santa Cruz, CA) or rabbit mAb against total Rap1 (2399; Cell Signaling Technology, Danvers, MA) and processed as described previously (37) with corresponding HRP-coupled secondary Ab and chemiluminescence reagents from GE Healthcare (Freiburg, Germany).

    Techniques: Expressing, Incubation, SDS Page, Western Blot, Control